지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
이용수
초록· 키워드
오류제보하기
Genomic DNA isolated from two Porphyra species, P. tenera and P. dentate from Wando located on the southern coast of Korean peninsula was amplified by PCR reaction. The amplified products were separated by agarose gel electrophoresis (AGE) with decamer primer and stained with ethidium bromide. The eight arbitrarily selected primers OPA-04, OPA-06, OPB-01, OPB-08, OPB-10, OPB-11, OPB-14 and OPC-10 generated the shared loci, polymorphic, and specific loci. The size of DNA bands varies from 100 bp to 2,200 bp. The complexity of the banding patterns varies dramatically between the primers and two Porphyra species. A total of 528 loci observed were identified in P. tenera and 443 in P. dentata: 22 polymorphic loci (4.2%) in P. tenera and 30 (6.8%) in P. dentata. 154 shared loci observed, the average 19.3 per primer, were identified in P. tenera and 143 loci, the average 17.9 per primer, in P. dentata species. The number of specific loci in P. tenera and P. dentata was 73 and 77, respectively. The average bandsharing value was 0.623±0.008 within P. tenera and 0.560±0.009 within P. dentata. The average bandsharing value between two Porphyra species was 0.408±0.004, ranged from 0.305 to 0.564. The dendrogram obtained by the eight primers indicates four genetic clusters. The genetic distance between two Porphyra species ranged from 0.076 to 0.627. The individual no. 02 of P. tenera was genetically closely related to no. 01 of P. tenera (genetic distance=0.082). Especially, two entities between the individual DENTATA no. 21 and DENTATA no. 19 of P. dentata showed the longest genetic distance (0.627) in comparison with other individuals used. In this study, RAPD-PCR analysis has revealed the significant genetic distance between two Porphyra species pairs (P＜0.001).
목차
Abstract
Introduction
Materials and Methods
Results and Discussion
Acknowledgements
References
참고문헌 (38)
참고문헌 신청
[학술지(정기간행물)] - Adams, R. P / 2000 / Systematics of the one seeded Juniperus of the eastern hemisphere based on leaf essential oils and random amplified polymorphic DNAs (RAPDs) / Biochem. Syst. Ecol 28 : 529 ~ 543
[학술지(정기간행물)] - Bartish, I. V / 2000 / Phylogenetic relationships and differentiation among and within populations of Chaenomeles Lindl. (Rosaceae) estimated with RAPDs and isozymes / Theor. Appl. Genet 101 : 554 ~ 563
[학술지(정기간행물)] - Bommineni, V. R / 1997 / Analysis of hybrids of durum wheat with Thinopyrum juncelforme using RAPD markers / Theor. Appl. Genet 95 : 757 ~ 763
[학술지(정기간행물)] - Callejas, C. / 1998 / Identification of Spanish barbel species using the RAPD technique / J. Fish Biol 53 : 208 ~ 215
[학술지(정기간행물)] - Esselman, E. J / 2000 / RAPD marker diversity within and divergence among species of Dendroseris (Asteraceae: Lactuceae) / Am. J. Bot 87 : 591 ~ 596
[학술지(정기간행물)] - Bartish, I. V / 2000 / Phylogenetic relationships and differentiation among and within populations of Chaenomeles Lindl. (Rosaceae) estimated with RAPDs and isozymes / Theor. Appl. Genet 101 : 554 ~ 563
[학술지(정기간행물)] - Bommineni, V. R / 1997 / Analysis of hybrids of durum wheat with Thinopyrum juncelforme using RAPD markers / Theor. Appl. Genet 95 : 757 ~ 763
[학술지(정기간행물)] - Callejas, C. / 1998 / Identification of Spanish barbel species using the RAPD technique / J. Fish Biol 53 : 208 ~ 215
[학술지(정기간행물)] - Esselman, E. J / 2000 / RAPD marker diversity within and divergence among species of Dendroseris (Asteraceae: Lactuceae) / Am. J. Bot 87 : 591 ~ 596
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UCI(KEPA) : I410-ECN-0101-2009-529-015613797