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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
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This study was conducted to investigate the effects of three different concentrations (6%, 8% and 10% final volume) of dimethyl sulfoxide (DMSO) on cryopreserved sperm of grass carp (Ctenopharyngodon idellus). Grass carp sperm was suspended in Kurokura extender #2 and equilibrated at 4℃ for 10 min. French straws (0.25 ml) of sperm were frozen from 4℃ to -4℃ at a rate of 4℃ min?¹ and then from -4℃ to -80℃ at a rate of 11℃ min?¹. The straws were kept at -80℃ for 10 min and finally stored in liquid nitrogen (-196℃). The cryopreserved sperm was thawed in a water bath at 40℃ for 30 sec and fertilization, hatching rate and larval malformation were compared with fresh sperm (control). The fertilization rate of post-thawed sperm was comparable (from 88.21% to 94.30%) to that of fresh sperm. However, hatching rate of all frozen sperm were significantly lower (P＜0.05) than that of control. Additionally, the larval abnormality rate of frozen sperm was significantly higher than that of fresh sperm. The results indicate that DMSO could affect the quality of cryopreserved sperm of grass carp, and a freezing program and a proper extender composition should be further studied.
목차
Abstract
Introduction
Materials and Methods
Results and Discussion
Acknowledgements
References
참고문헌 (27)
참고문헌 신청
[학술지(정기간행물)] - Alvarez, B / 2003 / High fry production rates using post-thaw silver carp (Hypophthalmichthys molitrix) spermatozoa under farming conditions / Aquaculture 220 : 195 ~ 201
[학술지(정기간행물)] - Babiak, I / 1997 / Cryopreservation of sperm of common carp carp, Cyprinus carpio L. / L. Aquacult. Res 28 : 567 ~ 571
[학술지(정기간행물)] - Bart, A.N / 1996 / Effects of sperm concentration and egg number on fertilization efficiency with channel catfish (Ictalurus punctatus) eggs and blue catfish (I. furcatus) spermatozoa / Theriogenology 45 : 673 ~ 682
[학술지(정기간행물)] - Basavaraja, A / 2004 / Cryopreservation of endangered mahseer (Tor khudree) spermatozoa: I. Effect of extender composition, cryoprotectants, dilution ratio, and storage period on post-thaw viability / Cryobiology 49 : 149 ~ 156
[학술지(정기간행물)] - Billiard, R / 1995 / Biology of sperm and artificial reproduction in carp / Aquaculture 129 : 95 ~ 112
[학술지(정기간행물)] - Babiak, I / 1997 / Cryopreservation of sperm of common carp carp, Cyprinus carpio L. / L. Aquacult. Res 28 : 567 ~ 571
[학술지(정기간행물)] - Bart, A.N / 1996 / Effects of sperm concentration and egg number on fertilization efficiency with channel catfish (Ictalurus punctatus) eggs and blue catfish (I. furcatus) spermatozoa / Theriogenology 45 : 673 ~ 682
[학술지(정기간행물)] - Basavaraja, A / 2004 / Cryopreservation of endangered mahseer (Tor khudree) spermatozoa: I. Effect of extender composition, cryoprotectants, dilution ratio, and storage period on post-thaw viability / Cryobiology 49 : 149 ~ 156
[학술지(정기간행물)] - Billiard, R / 1995 / Biology of sperm and artificial reproduction in carp / Aquaculture 129 : 95 ~ 112
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UCI(KEPA) : I410-ECN-0101-2009-529-015613762