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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
초록· 키워드
오류제보하기
To compensate the problems of chemical assay in detoxification of recalcitrant and a practical approach in selection of bioremediation bacteria, a simple and rapid toxicity evaluation system was constructed based on Lumbricus rubellus. Long term-culture and specific equipment are not necessary, and semi-quantitative analysis of toxicity at sub-lethal concentration is possible by measuring of dose-dependent increased yellowish secreted compounds. When the toxicity of endosulfan, its metabolites and structurally related chemicals were measured for 24 h, the results were coincided with previous reports. Toxicity was found in endosulfan, endosulfan sulfate, aldrin, and dieldrin, respectively. Rapid and economic selection of endosulfan-detoxifying bacteria was possible using our system. Klebsiella pneumoniae KE-1, K. oxytoca KE-8 and Pseudomonas sp. KS-2P, reported endosulfan degrading bacteria, ameliorated the endosulfan toxicity, whereas E. coli, B. subtilis and other bacteria failed to protect the toxicity of endosulfan in L. rubellus. Our results suggest that the constructed system is useful to selection of microorganism as well as toxicity evaluation against toxic recalcitrants.
목차
Abstract
실험방법
결과 및 고찰
요약
감사의 글
참고문헌
참고문헌 (26)
참고문헌 신청
Ait-Aissa, S., P. Pandard, H. Magaud, A. P. Arrigo, E. Thybaud and J. M. Porcher. 2003. Evaluation of an in vitro hsp70 induction test for toxicity assessment of complex mixtures: comparison with chemical analyses and ecotoxicity tests. Ecotoxicol. Enviro
An, Y. J. 2005. Assessing soil ecotoxicity of methyl tert-butyl ether using earthworm bioassay; closed soil microcosm test for volatile organic compounds. Environ. Pollut. 134, 181-186.
Awasthi, N., A. K. Singh, R. K. Jain, B. S. Khangarot and A. Kumar. 2003. Degradation and detoxification of endosulfan isomers by a defined co-culture of two Bacillus strains. Appl. Microbiol. Biotechnol. 62, 279-283.
Boyd, D. R., N. D. Sharma and C. C. R. Allen. 2001. Aromatic dioxygenase: molecular biocatalysis and applications. Curr. Opin. Biotechnol. 12, 564-573.
Bundy, J. G., D. J. Spurgeon, C. Svendsen, P. K. Hankard, J. M., Weeks, D. Osborn, J. C. Lindon and J. K. Nicholson. 2004. Environmental metabonomics: applying combination biomarker analysis in earthworms at a metal contaminated site. Ecotoxicology 13, 79
An, Y. J. 2005. Assessing soil ecotoxicity of methyl tert-butyl ether using earthworm bioassay; closed soil microcosm test for volatile organic compounds. Environ. Pollut. 134, 181-186.
Awasthi, N., A. K. Singh, R. K. Jain, B. S. Khangarot and A. Kumar. 2003. Degradation and detoxification of endosulfan isomers by a defined co-culture of two Bacillus strains. Appl. Microbiol. Biotechnol. 62, 279-283.
Boyd, D. R., N. D. Sharma and C. C. R. Allen. 2001. Aromatic dioxygenase: molecular biocatalysis and applications. Curr. Opin. Biotechnol. 12, 564-573.
Bundy, J. G., D. J. Spurgeon, C. Svendsen, P. K. Hankard, J. M., Weeks, D. Osborn, J. C. Lindon and J. K. Nicholson. 2004. Environmental metabonomics: applying combination biomarker analysis in earthworms at a metal contaminated site. Ecotoxicology 13, 79
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UCI(KEPA) : I410-ECN-0101-2009-470-015376271