지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
이용수
초록· 키워드
오류제보하기
Genomic DNAs were extracted from the muscle of twenty-two specimens of two shortnecked clam, Ruditapes philippinarum populations collected in Anmyeondo and Seocheon. Genetic differences within and between populations were analysed by random amplified polymorphic DNAs-polymerase chain reaction (RAPD-PCR) using twenty arbitrary decamer primers. Out of 20 primers, 6 generated a total of 1,111 major and minor RAPD bands from individuals of two sites, producing approximately 4.2 average polymorphic bands per primer in individuals from Anmyeondo and ranging in size from less than 50 to larger than 1,500 base pairs (bp). The electrophoretic analysis of RAPD products amplified showed moderate levels of similarity among the different individuals in Seocheon population. The average bandsharing values (BS value) of the samples within population from Anmyeondo ranged from 0.155 to 0.684, whereas it was 0.143~0.782 within population from Seocheon. The average BS value between individuals No. 13 and No. 14 from Seocheon was 0.782 which was higher than that of those from Anmyeondo. The single linkage dendrogram resulted from three primers (OPA-08, -09 and -20), indicating six genetic groupings composed of group 1 (No. 4, 8 and 10), group 2 (No. 18), group 3 (No. 2, 5 and 7), group 4 (No. 1, 3, 6, 9, 11, 12, 13, 14, 15 and 17), group 5 (16, 19 and 20) and group 6 (No. 21 and 22). In the Seocheon population, the individual No. 18 clustered distinctly from the others of this population. The observed genetic distance between the two populations from Anmyeondo and Seocheon was more than 0.209 (0.247 and 0.275). The shortest genetic distance (0.094) displaying significant molecular differences was between individuals No. 13 and No. 14. Especially, the genetic distance between individuals No. 22 and the remnants among individuals in two geographical populations was highest (0.275). This result illustrated that individual No. 22 is distinct from other individuals within two shortnecked populations. The different geographical features of two sites may have caused the genetic diversity in two shortnecked clam populations.
목차
Abstract
Introduction
Materials and Methods
Results
Discussion
Acknowledgements
References
참고문헌 (31)
참고문헌 신청
[학술지(정기간행물)] - / 1997 / Genetic variability of the wild diploid wheat Triticum urartu revealed by RFLP and RAPD markers 94 ( ) : 424 ~ 430
[학술지(정기간행물)] - / 1998 / Identification of Spanish barbel species using the RAPD technique 53 : 208 ~ 215
[학술지(정기간행물)] - / 1998 / Genetic changes in an Atlantic salmon population resulting from escaped juvenile farm salmon 52 : 118 ~ 127
[학술지(정기간행물)] - / 1999 / Assessment of genetic variation within Brassica campestris cultivars using amplified fragment length polymorphism and random amplification of polymorphic DNA markers 24 (4) : 433 ~ 440
[학술지(정기간행물)] - / 1993 / The use of RAPDs for the study of the genetic diversity of Schistosoma mansoni and Trypanosoma cruzi : 339 ~ 345
[학술지(정기간행물)] - / 1998 / Identification of Spanish barbel species using the RAPD technique 53 : 208 ~ 215
[학술지(정기간행물)] - / 1998 / Genetic changes in an Atlantic salmon population resulting from escaped juvenile farm salmon 52 : 118 ~ 127
[학술지(정기간행물)] - / 1999 / Assessment of genetic variation within Brassica campestris cultivars using amplified fragment length polymorphism and random amplification of polymorphic DNA markers 24 (4) : 433 ~ 440
[학술지(정기간행물)] - / 1993 / The use of RAPDs for the study of the genetic diversity of Schistosoma mansoni and Trypanosoma cruzi : 339 ~ 345
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UCI(KEPA) : I410-ECN-0101-2009-529-015356197