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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 1997.11
- 수록면
- 409 - 415 (7page)
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초록· 키워드
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The carp β-actin regulatory sequences and RSV/LTR promoter were tested whether they are functional to express linked structure gene (chloramphenicol acetyltransferase, CAT) in olive flounder (Paralichthys olivaceus) by determining the patterns of gene expression following intramuscular in vivo direct injection. The injection experiments with various concentrations of both pRSVCAT and pFV4CAT clearly revealed the effectiveness of DNA dosage on expression of CAT. The increase of CAT activity was linear in both plasmids, and maximal CAT activity was obtained with 100 ㎍ of pFV4CAT injection. The amounts of CAT expression with pFV4CAT-injected fish were higher than those with pRSVCAT-injected fish. CAT activity was readily detectable as early as one day after injection, slightly increased at day 2, and declined over time. Most amount of DNA intramuscularly injected into olive flounder muscles persisted extrachromosomally without showing any integrated or replicated form in vivo.
목차
Abstract
Introduction
Materials and methods
Results
Discussion
Acknowledgement
References
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UCI(KEPA) : I410-ECN-0101-2009-529-015350064