지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 1995.8
- 수록면
- 171 - 181 (11page)
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초록· 키워드
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Gonadosomatic index (GSI) of the crucian carp (Carassius auratus) was investigated to clarify annual reproductive cycle from February in 1993 to October in 1994. The values of GSI were high with individual variation from April to July which period was coincided with the breeding season of fish. The GSI was very low in August and September, when follicular atresia developed in the ovaries. GSI value began to increase in October and reached a peak around the following March, which indicated that ovarian follicles may grow during this period.
Human chorionic gonadotropin (HCG 10 IU), 17α, 20β - dihydroxyprogesterone (1 - 100 ㎍ / ml) and phorbol 12 - myristate - l3 - acetate (TPA, protein kinase C activator, 0.1 - 10 μM) induced germinal vesicle breakdown (GVBD), but 4α - phorbol 12, 13 - didicanoate (4α - PDD, phorbol ester analogue, 25 μM) did not induce germinal vesicle breakdown in the follicular oocytes. Prostaglandin F_(2a) (0.1 - 10㎍ / ml) and TPA (0.1 - 10μM) induced ovulation of the oocytes, but 4α - PDD (25 μM) did not induce ovulation of the follicles. 17α - hydroxyprogesterone production was examined from the isolated follicles to investigate the steroid production ability in the crucian carp ovaries. HCG (1 IU, 10 IU) and forskolin (adenylate cyclase activator, 0.1 - 10 μM) stimulated 17α - hydroxyprogesterone production. The time course of HCG (10 IU) and forskolin (10 μM) stimulated 17α - hydroxyprogesterone production within 3 hours, the elevated levels were maintained during the rest of the culture period.
The data indicates that cyclic AMP and protein kinase C may play important roles in the oocyte maturation and ovulation in crucian carp.
Human chorionic gonadotropin (HCG 10 IU), 17α, 20β - dihydroxyprogesterone (1 - 100 ㎍ / ml) and phorbol 12 - myristate - l3 - acetate (TPA, protein kinase C activator, 0.1 - 10 μM) induced germinal vesicle breakdown (GVBD), but 4α - phorbol 12, 13 - didicanoate (4α - PDD, phorbol ester analogue, 25 μM) did not induce germinal vesicle breakdown in the follicular oocytes. Prostaglandin F_(2a) (0.1 - 10㎍ / ml) and TPA (0.1 - 10μM) induced ovulation of the oocytes, but 4α - PDD (25 μM) did not induce ovulation of the follicles. 17α - hydroxyprogesterone production was examined from the isolated follicles to investigate the steroid production ability in the crucian carp ovaries. HCG (1 IU, 10 IU) and forskolin (adenylate cyclase activator, 0.1 - 10 μM) stimulated 17α - hydroxyprogesterone production. The time course of HCG (10 IU) and forskolin (10 μM) stimulated 17α - hydroxyprogesterone production within 3 hours, the elevated levels were maintained during the rest of the culture period.
The data indicates that cyclic AMP and protein kinase C may play important roles in the oocyte maturation and ovulation in crucian carp.
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