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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 1993.8
- 수록면
- 221 - 233 (13page)
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Cell size, DNA content, chromosome and PCR-based mitochondrial 12S rRNA gene analyses were conducted to obtain basic informations for genetic stock identification of spotted flounder (Verasper variegatus) from Yeocheun, Korea.
The mean erythrocytic and nuclear volumes of spotted flounder were 211.10㎛³ and 23.03 ㎛³, respectively. The haploid DNA content of this species was 0.79 pg/cell which correspond to 46.5 % of carp and to 22.6 % of mammals. Spotted flounder had the 2n=46 acrocentric chromosomes but no heteromorphic sex chromosomes was found. Mitochondrial DNA gene for 12S ribosomal RNA was amplified by polymerase chain reaction (PCR) and the PCR products were subjected to digestion with 15 restriction endonucleases. Restriction enzyme analyses revealed that Ava Ⅰ , Mae Ⅱ, Sma Ⅰ and Xba Ⅰ had one restriction site in the mitochondrial 12S rRNA gene segment of spotted flounder, while Mae Ⅰ had two. Segments of 12S rRNA gene from mitochondria in spotted flounder were sequenced and compared with channel catfish and human as controls. The 12S rRNA gene of this species was more similar to that of channel catfish than to human's.
The mean erythrocytic and nuclear volumes of spotted flounder were 211.10㎛³ and 23.03 ㎛³, respectively. The haploid DNA content of this species was 0.79 pg/cell which correspond to 46.5 % of carp and to 22.6 % of mammals. Spotted flounder had the 2n=46 acrocentric chromosomes but no heteromorphic sex chromosomes was found. Mitochondrial DNA gene for 12S ribosomal RNA was amplified by polymerase chain reaction (PCR) and the PCR products were subjected to digestion with 15 restriction endonucleases. Restriction enzyme analyses revealed that Ava Ⅰ , Mae Ⅱ, Sma Ⅰ and Xba Ⅰ had one restriction site in the mitochondrial 12S rRNA gene segment of spotted flounder, while Mae Ⅰ had two. Segments of 12S rRNA gene from mitochondria in spotted flounder were sequenced and compared with channel catfish and human as controls. The 12S rRNA gene of this species was more similar to that of channel catfish than to human's.
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UCI(KEPA) : I410-ECN-0101-2009-529-015329205