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자료유형
학술저널
저자정보
저널정보
대한기계학회 Journal of Mechanical Science and Technology Journal of Mechanical Science and Technology Vol.19 No.11[Special Edition]
발행연도
2005.11
수록면
2,122 - 2,126 (5page)

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Major challenges in the field of electrochemical DNA hybridization biosensors are the immobilization of DNA and the detection of hybridization signals. The method of DNA immobilization using the nano-patterning machine and detection for DNA hybridization signals has been proposed. Here, two gold electrodes were deposited on SiO₂ layer and the gap between the electrodes was fabricated by electron beam lithography. 3-aminopropyltriethoxysilane (APTES) solution was selectively treated to immobilize the amino-modified oligonucleotides onto the SiO₂ layer between the electrodes. The recognition of DNA hybridization was accomplished by metallic aggregation of nano-particles. The results showed that DNA is immobilized with nanometer scales and the method for detecting hybridization signals is useful. The experimental results were verified by I-V curves. The conductance between two electrodes changed with the density of the Au-nanoparticles immobilized onto the oxide layer. These results can be applied to the DNA chip and the multi-functional sensors which will be researched in the further study.

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Abstract

1. Introduction

2. Materials and Methods

3. Results and Discussion

4. Conclusion

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