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초록· 키워드

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Wood chips of Korean Quercus sp. was steam exploded with the addition of 0.2% sulfuric acid at 215℃ for 3 to 6 minutes, and subsequently was hydrolyzed by Celluclast™ (and Novozym 188™) to produce hydrolysates contaning sugars. The hydrolysates with 3 to 5%(w/v) glucose (at maximum 8%) was not adequate for fermentation concerning subsequent ethanol recovery by distillation. Hence, it was concentrated at maximum 70°C temperature by vacuum evaporation to higher than 14% glucose concentration.
However, because of certain inhibitory materials probably produced during the explosion, hydrolysis and sterilization, hydrolysates containing higher than 80g/L glucose was not fermented properly. Yeast strain adaptation to concentrated hydrolysates, detoxification treatment with charcoal and lime were tested for improved fermentation to find some limited effects, and the charcoal treatment was found to be the most effective one. Surprisingly, sterilization at below 60℃ temperature for 2 hours instead of conventional 120℃, 15 minutes make the hydrolysates fermentation possible up to 140 g/L glucose concentration. Hence, it is assumed that certain toxic materials are produced additionally during the sterilization of concentrated hydrolysates.
The inhibitory effects of toxic materials produced during pretreatment and sterilization including lignin and sugar degradation products (such as vanillin, p-hydroxy bezaldehyde, syringaldehyde and furfural) were tested to identify those which could be prohibitive. It was found that the lignin degradation products are more toxic than sugar degradation products.

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UCI(KEPA) : I410-ECN-0101-2009-563-018150935