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자료유형
학술저널
저자정보
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한국식품영양과학회 Journal of Food Science and Nutrition Journal of Food Science and Nutrition Vol.1 No.1
발행연도
1996.6
수록면
80 - 86 (7page)

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Parathyroid hormone(PTH) is known to stimulate bone resorption and to inhibit bone collagen synthesis. In contrast, as the evidence of stimulation of bone formation by PTH has recently been observed, the study on the role of PTH involved in osteoporosis draws remarkable attention. This study has dealt with the role of alkaline phosphatase(AP), a marker enzyme for bone formation and osteoblast action. Animals(BALB/c mice) were divided into three dietary groups(high and medium Ca and Ca-free) and hormones including PTH, calcitonin(CT), cholecalciferol(vitamin D) were i.p. injected. AP in the serum and liver was measured using Sigma 221 alkaline buffer solutions containing 9mM of p-nitrophenyl phosphate. Enzyme was reacted at 37℃ for 10 minutes and the reaction was stopped by 1.8ml of 0.1N NaOH and measured at 410㎚. We found that serum and liver AP activity was increased by low dietary Ca. Compared to the control, and serum AP activity was enhanced by PTH and vitamin D regardless of the levels of dietary Ca. On the other hand, liver AP activity was inhibited by PTH and vitamin D at all levels of dietary Ca. CT inhibited the action of PTH and vitamin D in the serum. But, the inhibition of PTH and vitamin D action by CT was not observed in the liver, unlike in the case of serum.

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Abstract

INTRODUCTION

MATERIALS AND METHODS

RESULTS

DISCUSSION

ACKNOWLEDGEMENTS

REFERENCES

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