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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2004.9
- 수록면
- 1,429 - 1,434 (6page)
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초록· 키워드
오류제보하기
Immunoassay is one of the important analytical methods for clinical diagnoses and biochemical studies, but needs a long time, troublesome procedures and expensive reagents. In this study, therefore, we propose the micro filter chip with microbe ads for immunoassay, which has pillar structures. The advantage of the proposed micro filter chip is to use simple fabrication process and cheap materials. The mold was made by the photolithography technique with Si wafer and negative photoresist SU-8. The replica was made of PDMS, bonded on the pyrex glass. The micro filter chip consists of inlet channel, filter chamber and outlet channel. HBV (Hepatitius B virus) monoclonal antibody (AgI) labeled with biotin were immobilized onto streptavidin coated beads of 30-50㎛ size. Fluorescein isothiocyanate (FITC)-labeled HBV monoclonal antibody (Ag8) was used to detect HBsAg (Hebatitis B virus surface Antigen), and fluorescence intensity was monitored by epi-fluorescence microscope. In this study, the immune response of less than 30 min was obtained with the use of 100 ㎖ of sample.
목차
Abstract
1. 서론
2. 마이크로 바이오칩의 설계
3. 바이오칩 제작 및 결과
4. 면역분석 실험
5. 결론
참고문헌
참고문헌 (8)
참고문헌 신청
[학술지(정기간행물)] - / 2003 / Analysis of Fully Developed Mulitilayer Flow in Microchannel with a Rectangular Cross Section 27 (5) : 644 ~ 654
[학술지(정기간행물)] - / 2003 / Predictions of Microscale Separated Flow using Langmuir Slip Boundary Condition 27 : 1097 ~ 1104
[학술지(정기간행물)] - / 1999 / Microsystem Technology in Chemistry and Life Sciences : 10 ~
[학술지(정기간행물)] - / 2001 / A Rapid Diffusion Immunoassay in a T-sensor 19 : 461 ~ 465
[학술지(정기간행물)] - / 2001 / Determination of Carcinoembryonic Antigen in Human Sera by Integrated Bead-Bed Immunoasay in a Microchip for Cancer Diagnosis 73 : 1213 ~ 1218
[학술지(정기간행물)] - / 2003 / Predictions of Microscale Separated Flow using Langmuir Slip Boundary Condition 27 : 1097 ~ 1104
[학술지(정기간행물)] - / 1999 / Microsystem Technology in Chemistry and Life Sciences : 10 ~
[학술지(정기간행물)] - / 2001 / A Rapid Diffusion Immunoassay in a T-sensor 19 : 461 ~ 465
[학술지(정기간행물)] - / 2001 / Determination of Carcinoembryonic Antigen in Human Sera by Integrated Bead-Bed Immunoasay in a Microchip for Cancer Diagnosis 73 : 1213 ~ 1218
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UCI(KEPA) : I410-ECN-0101-2009-550-014427294