지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
이용수
초록· 키워드
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This study was performed in order to recognize the identifications of the glycoproteins containing oligosaccharides in human gingiva
After made paraffin sections of human gingiva at 4㎛, the sections were incubated with 7 lectins (UEA-I, BS-I, SBA, DBA, WGA, PNA, PNA after neuraminidase treated, Con-A) In order to increase specificity of reactions, the sections were applicated with ABC system, And then the sections were incubated with DAB and were counterstained with hematoxylin Using the same sections, the sections were done H-E and PAS stains
In WGA, DBA and Con-A, plasma membranes of the layers of all epithelium and connective tissue were stained In BS-I, In the epithelium of marginal gingiva, plasma membranes of upper layer of the Spinous cell layer and granular cell layer were stained And in epithelium of sulcular gingiva, plasma membranes of the all spinous cell layer and granular cell layer were stained in SBA, Plasma membranes of the granular cell layer were stained In PNA, In the epithelium of marginal gingiva, plasma membranes of the basal cell layer and lower layer of spinous cell layer were stained. But lectin reactions were not occurred in the sulcular gingiva. In PNA treated neuraminidase, plasma membranes of the all epithelial layer except basal cell layer membranes especially cytoplasms of upper layer at the sulcular gingiva and connective tissue were reacted
1. By the above results, authors could know the identifications of oligosaccharides existing glycoproteins in the human gingiva.
1) All epithelial layer, α-D-N-Acetyl-Galactosamine, Sialic acid, D-Glucosamine, α-D-Mannose
2) Basal cell layer, Galactose-β-(1-3)-N-Acetyl-Galactosamine
3) Spinous cell layer; α-D-Galactose, Galactose-β-(1-3)-N-Acetyl-Galactosamine
4) Granular cell layer, α-D-Galactose
5) Connective tissue; α-D-N-Acetyl-Galactosamine, Sialic acid, β-(1-4)-D-Acetyl-Glucosamine, α-D-Glucosamine, α-D-Mannose
2. The Galactose-β-(1-3)-N-Acetyl-Galactosamine was not existed in the basal cell layer and spinous cell layer in the sulcular gingiva
After made paraffin sections of human gingiva at 4㎛, the sections were incubated with 7 lectins (UEA-I, BS-I, SBA, DBA, WGA, PNA, PNA after neuraminidase treated, Con-A) In order to increase specificity of reactions, the sections were applicated with ABC system, And then the sections were incubated with DAB and were counterstained with hematoxylin Using the same sections, the sections were done H-E and PAS stains
In WGA, DBA and Con-A, plasma membranes of the layers of all epithelium and connective tissue were stained In BS-I, In the epithelium of marginal gingiva, plasma membranes of upper layer of the Spinous cell layer and granular cell layer were stained And in epithelium of sulcular gingiva, plasma membranes of the all spinous cell layer and granular cell layer were stained in SBA, Plasma membranes of the granular cell layer were stained In PNA, In the epithelium of marginal gingiva, plasma membranes of the basal cell layer and lower layer of spinous cell layer were stained. But lectin reactions were not occurred in the sulcular gingiva. In PNA treated neuraminidase, plasma membranes of the all epithelial layer except basal cell layer membranes especially cytoplasms of upper layer at the sulcular gingiva and connective tissue were reacted
1. By the above results, authors could know the identifications of oligosaccharides existing glycoproteins in the human gingiva.
1) All epithelial layer, α-D-N-Acetyl-Galactosamine, Sialic acid, D-Glucosamine, α-D-Mannose
2) Basal cell layer, Galactose-β-(1-3)-N-Acetyl-Galactosamine
3) Spinous cell layer; α-D-Galactose, Galactose-β-(1-3)-N-Acetyl-Galactosamine
4) Granular cell layer, α-D-Galactose
5) Connective tissue; α-D-N-Acetyl-Galactosamine, Sialic acid, β-(1-4)-D-Acetyl-Glucosamine, α-D-Glucosamine, α-D-Mannose
2. The Galactose-β-(1-3)-N-Acetyl-Galactosamine was not existed in the basal cell layer and spinous cell layer in the sulcular gingiva
목차
Ⅰ. 서론
Ⅱ. 재료 및 방법
Ⅲ. 결과
Ⅳ. 고찰
Ⅴ. 요약
참고문헌
Abstract
Explanation of Figures
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